The recommended RNA input is 100 ng for panels with <400 genes and 50 ng for panels >400 genes. The input might need to be modified depending on RNA quality
For FFPE or degraded RNA samples the recommended input is 300 ng.
The samples should be diluted to between 20-30 ng/uL in RNase-free water. Please contact us for required amount and volume for your experiment.
Samples should be delivered in eppendorf tubes or PCR strips and clearly labeled directly on the lid of the tube.
RNA concentration and purity should be measured using a NanoDrop or other spectrophotometer. The A260/A280 ratio should be 1.7–2.3 and the A260/A230 ratio 1.8–2.3. If you want NanoDrop testing of your samples, please contact us.
We also recommend evaluating the integrity of the samples using a Bioanalyzer. Partially degraded samples can still be analyzed on the nCounter but the input amount might have to be increased.
For FFPE or highly degraded samples it is recommended that at least 50% of the RNA fragments are longer than 300 nucleotides. If you want Bioanalyzer analysis of your samples, please contact us.