Welcome to a Joint LLC Seminar on the 8^th of June, at 15.15 in Rydbergsalen: Kishan Dholakia, University of St Andrews and University of Adelaide.

Kishan Dholakia will talk on the topic “Structured Light Sheet Microscopy: advances in application and analysis”.

Abstract:

We have seen great advances in photonics-based methods for imaging. However, whilst improvements have been seen in beating the diffraction limit, obtaining wide field imaging and imaging through scattering media remain a challenge. I will describe approaches that tackle this issue for light sheet imaging or selective plane illumination microscopy.
For the goal of a wider field of view, I will describe the use of shaped fields namely propagation invariant (‘non-diffracting’) light fields and complex beam shaping for light sheet imaging. Propagation invariant light fields retain their transverse intensity profile upon propagation. More recent advances using attenuation compensation of fields [1], three photon light sheet imaging [2] and new sample mounting procedures [3] using acoustic confinement will be highlighted.
More recently we have explored the use of deep learning. We have developed an unsupervised approach that is trained on a single light sheet volume dramatically reducing the image data needed [4]. This can be combined with shaped light for enhanced imaging performance.
[1] J. Nylk et al. “Light-sheet microscopy with attenuation-compensated propagation-invariant beams”, Science Advances 4, eaar4817 (2018)
[2] A. Escobet-Montalbán, F. M. Gasparoli, J. Nylk, P. Liu, Z. Yang, and K. Dholakia, "Three-photon light-sheet fluorescence microscopy," Opt. Lett. 43, 5484-5487 (2018)
[3] Z. Yang et al., “Light sheet microscopy with acoustic sample confinement”, Nat. Comm. 10, 699 (2019)
[4] Wijesinghe et al., “Experimentally unsupervised deconvolution for light-sheet microscopy with propagation-invariant beams”, Light, Science and Applications (Nature) 11, 319 (2022)