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SpatialOmics@LU

Digital Spatial Profiling

NanoString’s GeoMx Digital Spatial Profiler combines standard immunofluorescence with digital optical barcoding to perform highly multiplexed, spatially resolved expression analysis of both protein and RNA in FFPE or fresh frozen tissue sections.

The GeoMx DSP instrument first images the tissue section with up to four fluorescent stains to visualize the tissue morphology and to guide the selection of regions of interest (ROI) for profiling of RNA or protein expression. The same tissue slide is also stained with RNA probes or antibodies coupled to photocleavable oligonucleotide tags that are released when the selected ROIs are sequentially exposed to UV light. The tags are then quantitated in the Nanostring nCounter instrument and mapped back to the tissue location, revealing the spatial expression patterns of the tissue.


Go to Nanostrings website to read more about the technology or contact us if you have any questions!


We plan to start offering the services in May - June of 2020.

Page Manager: Lina Olsson

Contact

SpatialOmicsLU@immun.lth.se

Lina Olsson
Research engineer
+46 46 222 15 42

 

Visiting address:
The Department of Immunotechnology is on floor 4 of building 406 at Medicon Village (Scheelevägen 2) in Lund.