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SCIBLU

Lund University

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Quality Control

DNA and RNA concentration

NanoDrop is used to measure concentration and purity of nucleic acids before starting a microarray experiment. The concentration of nucleic acid is dependent on what array to be used. OD260/280 should be close to 2.0 for RNA dissolved in water and 1.8 for DNA. OD260/230 should preferably be above 2. A lower ratio might indicate contamination, such as GITC or phenol residues from extraction. A low OD260/230 reading can also occur as an artefact in samples with low concentration (< 50 ng/µl).

RNA quality

Agilent 2100 Bioanalyzer is used to analyze the integrity of nucleic acids. Total RNA samples can be processed on either a Nano chip, Pico chip or a Small RNA chip, depending on the sample amounts and concentrations. The RNA Integrity Number (RIN) software algorithm allows the classification of total RNA, based on a numbering system from 1 to 10, with 1 being the most degraded and 10 being the most intact. Total RNA for expression studies needs to be of high and even quality. 

Page Manager: Lina Olsson

Contact

sciblu@immun.lth.se

Lina Olsson
Research engineer
+46 46 222 15 42

 

Visiting address:
The Department of Immunotechnology is on floor 4 of building 406 at Medicon Village (Scheelevägen 2) in Lund.

 

Visitors are kindly asked to book an appointment