Proteomics Services

The proteomics platform is part of the SWEGENE centre for integrative biology at Lund University (SCIBLU). The aim is to provide access for the large medical, natural science and technology research communities to a resource centre for protein analysis and identification. This encompasses giving access to, and providing education in the whole range of techniques from sample preparation to computer analysis of the results.  The centre has been equipped with integrated proteome analysis systems which consist of:  2D gel and HPLC systems, gel evaluation and matching software, robotic spot cutter, digester and target loader, MALDI mass spectrometer for protein fingerprinting and HPLC-MS/MS mass spectrometer for sequencing and protein modification analysis.  This is co-ordinated by a laboratory information system for sample tracking (Proteios) and analysis. Two analysis routes are offered. A full-service in which the centre carries out all of the wet-lab work and the customer only has to analyse the data. The alternative is a do-it-yourself laboratory where customers carry out most of the wet-lab work themselves and just provide the samples for mass spectrometry analysis.

The Centre can provide assistance in the form of:

- Hands-on wet-lab and dry-lab training in basic proteomics techniques
- Performing MALDI, Electrospray MS and MS/MS analyses.
- Running large-scale 2D-gel based experiments.
- Running ICAT/iTRAQ/SILAC HPLC-MS experiments and data analysis
- Verified and tested protocols for the different steps in a proteomics experiment.
- Software training and access to analysis tools.
- Access to gel/autoradiogram scanner and image analysis software.
- Option for the researcher to conduct the experiments in the Centre's laboratory.
- Under certain agreements a complete service including all laboratory analyses.

A researcher therefore has the option to e.g. learn the technique at the Centre in a course orworkshop, and then to perform the experiments in his or her own laboratory analysis using the protocols provided by the Centre. The researcher can then return to the Center for gel scanning or mass spectrometry analysis. The subsequent data analysis can be often be carried out in the home laboratory using the web based software.