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SCIBLU

Lund University

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Sample Requirement

OncoScan CNV
  • The OncoScan CNV assay enables detection of copy number variations, loss of heterozygosity, ploidy, break point determination, mosaicism, clonal heterogeneity, and chromotripsis in tumour samples. The OncoScan CNV assay has been shown to perform well with highly degraded DNA, such as FFPE tumour samples and with low inputs of DNA.
  • The OncoScan CNV assay is set-up for 23 samples and a positive control.
  • FFPE DNA must be extracted and handled according to recommendations in the OncoScan manual (pages 28-29 and 120-126).
  • At least 15µl of each sample, at a concentration of 12ng/µl should be delivered in a 96-well PCR-plate. Plate orientation A1-12, C1-12, etc. Samples must be eluted and diluted in low EDTA 1xTE buffer. Do NOT use water.
  • The sample concentration must be determined using a dsDNA specific quantification method. We strongly recommend using the PicoGreen assay or Qubit dsDNA quantification (Life Technologies). UV absorbance or NanoDrop should not be used, as it will overestimate the DNA concentration.
  • SCIBLU genomics will not make any further quality tests of the samples.


CytoScan HD
  • The CytoScan HD assay can detect known and novel chromosome aberrations across the entire human genome. It features whole-genome coverage, to detect gene-level, copy-neutral LOH, uniparental isodisomy, and regions identical-by-descent, in addition to low-level mosaicism and sample heterogeneity
  • DNA must be extracted and handled according to recommendations in the CytoScan HD Assay manual (pages 8-10).
  • The CytoScan HD assay is set-up for 100 samples.
  • At least 12ul of each DNA sample at a concentration of 50ng/ul should be delivered in a 96-well PCR plate, plate orientation A1-12, C1-12, etc, together with a gel-image showing high quality DNA with a major gel band at 10-20 kbp.
  • SCIBLU genomics will not make any further quality tests of the samples


DMET - Drug Metabolism
  • The DMET Plus assay is capable of processing complex genetic markers that influence the metabolism of commonly prescribed drugs and that are not assayed by conventional microarray SNP methods due to the presence of pseudogenes or other closely related genomic sequences.
  • The DMET Plus assay is set-up for 45 samples.
  • Please read the DMET manual for instructions on sample handling.
  • At least 17 ul of DNA at a concentration of 60 ng/µl should be delivered in a 96-well PCR plate together with a gel-image showing high quality DNA. Samples should be diluted in 1xTE buffer. Do NOT use water.
  • The sample concentration must be determined using a dsDNA specific quantification method. We strongly recommend using the PicoGreen assay or Qubit dsDNA quantification (Life Technologies). UV absorbance or NanoDrop should not be used, as it will overestimate the DNA concentration.
  • SCIBLU genomics will not make any further quality tests of the samples.


Sample delivery

Samples should be transported on dry ice to the delivery address. Please contact us before sending your samples to make sure that someone will receive the package. Send the samples to the following address:

Lunds Universitet
SCIBLU Genomics Microarray facility
Inst. för Immunteknologi (406)
Medicon Village, byggnad 402A
Scheelevägen 8
SE-223 63 Lund

Page Manager: Lina Olsson